Asian Journal of Biotechnology and Genetic Engineering https://journalajbge.com/index.php/AJBGE <p style="text-align: justify;"><strong>Asian Journal of Biotechnology and Genetic Engineering</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGE/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGE includes but not restricted to all aspects of Biotechnology, Genetics, Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, etc. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> en-US contact@journalajbge.com (Asian Journal of Biotechnology and Genetic Engineering) contact@journalajbge.com (Asian Journal of Biotechnology and Genetic Engineering) Wed, 19 Aug 2020 08:03:59 +0000 OJS 3.1.1.4 http://blogs.law.harvard.edu/tech/rss 60 Genetic Profiling of Cynodon dactylon Species Using ISSR Markers and Its Pharmacological Activities https://journalajbge.com/index.php/AJBGE/article/view/30080 <p><strong>Aim:</strong> <em>Cynodon dactylon </em>is a grass variety of the tropical and sub-tropical region belonging to the family Poaceae. In this study genetic profiling of 3 different varieties of <em>Cynodon dactylon </em>were done using ISSR primers.</p> <p><strong>Methodology:</strong> Polymorphism patterns were observed by amplifying DNA with 8 sets of primers such as OPA16, OPB11, OPC06 etc. PyElph tool was used to understand the genetic similarity of the samples and accordingly the relatedness was shown by dendrogram construction by DendroUPGMA. The grass samples were also used to check their antibacterial activity against potential pathogenic bacteria like <em>Pseudomonas aeruginosa, Salmonella typhi, Chrysobacterium gleum etc. </em>The plant species had showed a very satisfactory inhibition against the bacteria. <em>In vitro</em> anti-diabetic activity of <em>Cynodon dactylon </em>was checked by calculating its ability to inhibit the enzyme alpha-amylase and the anti-inflammatory activity was carried out by calculating the inhibition percentage of albumin degradation.</p> <p><strong>Outcome: </strong>All these experiments were done to study the pharmacological property as well as to gather knowledge on the genetic make-up of <em>Cynodon dactylon. </em>Overall, the final outcome supports the ethanobotanical claim of this plant.</p> Chandrika S. Tanthry, Saswati Mandal, S. D. Shruthi ##submission.copyrightStatement## https://journalajbge.com/index.php/AJBGE/article/view/30080 Wed, 19 Aug 2020 00:00:00 +0000 Bar Coding and Genetic Diversity Analysis of Millet Crop Species of Elusine Genus Using RAPD Markers https://journalajbge.com/index.php/AJBGE/article/view/30081 <p><strong>Aim:</strong> Millets are a group of highly variable small seeded grasses, grown around the world as cereal crops or grains for fodder and human food. In this study, screening of crop primers with 8 genotypes of millet crop species belonging to genus <em>Panicum, Eleusine,</em> <em>Echinochloa</em> and <em>Brachiaria</em> was done. Main objective was to understand the genetic diversity among 7 millet crops varieties and a wild grass using RAPD markers by targeting nuclear gene. To find out the relationship between species of the same genus based on RAPD markers and to construct phylogenetic tree using PyElph 1.4 and Past326b softwares.</p> <p><strong>Methodology:</strong> PCR analysis was done using the selected RAPD to identify the polymorphic loci between the 8 genotypes. Primers OPC06, OPD13, OPW04, OPC18 were selected for analysis based on the band patterns, for further RAPD analysis. Bar Coding and Sequencing was done for validation of species with the help of softwares BLAST and Clustal OMEGA. Phylogenetic tree was constructed using all 4 primers with the help of software tools PyElph 1.4 and Past326b. The point parameter, which guided the joining linkage rule, is unweighted pair group average (UPGMA) and the genetic distance was computed from raw data.</p> <p><strong>Outcome: </strong>Among the 4 primers used, OPW04 and OPD13 showed maximum scoring of bands based on the phylogeny were established between the crop species of <em>Eleusine</em> species and the Indian Goose grass. The dendrogram showed that the varieties could be grouped into clusters. The sample C (<em>Elusine indica</em>), D (<em>Elusine coracana</em>) is diverged from the same root and C, D and G (Indian goose grass) are from the same root. Sample G is found to be very closely related to the C and D.</p> Aarti R. Desai, B. Ramya, S. D. Shruthi ##submission.copyrightStatement## https://journalajbge.com/index.php/AJBGE/article/view/30081 Wed, 19 Aug 2020 00:00:00 +0000 Curative, Suppressive and Prophylactic Phyto-Medicinal Therapy/Synergistic Efficacy of Alstonia boonei/Capsicum frutescens Ethanolic Extracts against Plasmodium berghei (NK 65)/Salmonella typhi (ATCC 35723) Infected Swiss Albino Mice https://journalajbge.com/index.php/AJBGE/article/view/30082 <p>The purpose of this research work is to evaluates the medicinal activity of <em>Alstonia boonei and Capsicum frutescens</em> extracts for Prophylactic, Curative &amp; Suppressive phytomedicinal therapy against&nbsp; <em>Plasmodium berghei </em>(NK 65)/<em>Salmonella typhi </em>(ATCC 35723) Infected Swiss Albino Mice<strong>.</strong> <em>Alstonia boonei</em> belongs to the Apocynaceae family and has several medicinal properties, for the treatment of various diseases while <em>Capsicum frutescens</em> is a tropical plant, an important agricultural crop and a popular African vegetables. The stem bark of <em>Alstonia boonei</em> and fruit of <em>Capsicum frutescens</em> were collected from Adekunle Ajasin University’s reserve forest. Both plant samples were authenticated in the herbarium unit of Forest Research Institute of Nigeria (FRIN) with identification number FHI 109806,109872 respectively. <em>Plasmodium berghei</em> (NK 65) and <em>Salmonella Typhi </em>(ATCC 35723) from the Institute of Advanced Medical Research and Training (IAMRAT), College of Medicine, University of Ibadan, Nigeria. The animals were acclimatized for two weeks and fed equally on rat chow and water throughout the period of the research work. A standard inoculum of 1×10<sup>7</sup> of parasitized erythrocytes from a donor mouse and 1x10<sup>-6</sup> innoculum size of Salmonella typhi in volumes of 0.2 ml were used to infect the experimental animals intra-peritoneally. The suppressive antimalarial test also called“Test on Early Malaria Infection” was used in this study. Twenty five Male Swiss albino mice weighing between 18-20g were obtained from the animal house, the experimental&nbsp; animal were divided into five groups of five mice each, which were inoculated with the parasite and the organism (<em>Plasmodium berghei</em>/<em>Salmonella typhi</em>) on the first day of the experiment (day 1). 72 hours. Group’s 1-3 mice received 100, 200 and 400mg/kg body weight of the extract per day orally. While the 4<sup>th</sup> group served as the positive control received 10mg/kg Chloroquine/Ciprofloxacin orally, 5<sup>th</sup> group received 0.2ml distilled water and served as negative control for the same period. Result obtained on observed the establishment of malaria and typhoid infection by administration of treatments dosage with different concentration of <em>Alstonia boonei</em> and <em>Capsicum frutescens</em>. The synergism of <em>Alstonia boonei</em> stem bark and <em>Capsicum frutescens</em> extract with significant (P&lt; 0.05) dose dependent reduction in percentage parasitemia level at the three (100, 200, and 400) mg/kg doses, While P. <em>berghe</em>i parasitaemia was the highest, Chloroquine /Ciprofloxacin, a significant weight loss compared to the other mice groups, suggesting that weight loss is a key criterion in this model. The synergism of <em>Alstonia boonei</em> of stem bark and <em>Capsicum frutescens friut </em>&nbsp;extracts exhibits a significant curative, suppressive and prophylactic potency against <em>Plasmodium berghei </em>(NK 65)/<em>Salmonella typhi </em>(ATCC 35723) in infected mice as demonstrated by the reduction in the level of parasitaemia dose dependently. It is evident based on these findings that <em>Alstonia boonei / Capsicum frutescens</em> possess promising and potent antimalarial and anti-typhoid potential which justifies its usage in folk medicine for the management of malaria and typhoid infection.</p> Oludare Temitope Osuntokun, Isaac Tolulope Faniyi ##submission.copyrightStatement## https://journalajbge.com/index.php/AJBGE/article/view/30082 Wed, 19 Aug 2020 00:00:00 +0000