Asian Journal of Biotechnology and Genetic Engineering <p style="text-align: justify;"><strong>Asian Journal of Biotechnology and Genetic Engineering</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGE/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGE includes but not restricted to all aspects of Biotechnology, Genetics, Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, etc. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> en-US (Asian Journal of Biotechnology and Genetic Engineering) (Asian Journal of Biotechnology and Genetic Engineering) Sat, 16 Oct 2021 09:39:28 +0000 OJS 60 Spores and Extracts of Entomopathogenic Fungal Isolate (Paecilomyces formosus) as Potential Biolarvicide of Anopheles Mosquitoes <p><strong>Introduction: </strong><em>Paecilomyces formosus</em> is a geographically widespread entomopathogenic fungus that produces infectious conidia against <em>Anopheles </em>mosquito larvae, which curtail the uprising resistance of mosquitoes against synthetic insecticides. These mosquitoes are known vectors of human and animal pathogens, millions of people are killed by mosquito-borne diseases every year such as malaria, dengue, chikungunya, Zuka, yellow fever, encephalitis and filariasis.&nbsp;</p> <p><strong>Aim:</strong> This study investigated the spores and extract sourced from entomopathogenic (<em>Paecilomyces formosus</em>) fungal isolates as potential biolarvicide of <em>Anopheles</em> mosquitoes.</p> <p><strong>Methods: </strong>The conidia and extract bioassays were conducted according to WHO-2005 protocol with slight modification. The most active extract ethylacetate was characterized using Gas Chromatography-Mass Spectroscopy.</p> <p><strong>Results:</strong> From the conidia bioassay, The LC<sub>50 </sub>mortality of the larvae was found to be 1.4×10<sup>4</sup> conidia ml<sup>-1</sup> at 24 hrs 6.1×10<sup>5</sup> conidia ml<sup>-1</sup> at 48hrs 8.8×10<sup>4</sup> conidia/ml at 72 hrs. Solvents used for the extract bioassay includes; Diethyl-ether, Chloroform and Ethyl-acetate of which, Ethyl-acetate extract is found to be most active (LC50s; 101.5 μg/ml, 735.6 μg/ml, 769.0 μg/ml after 48-hours post exposure time.</p> <p>Gas Chromatography-Mass Spectroscopic analysis of ethyl-acetate extract showed 6 major compounds (R.T) 3, 4-Altrosan (9.14), I, 6-anhydro-β-glucopyranose (9.30), Pentanoic acid (10.97), methylpropandioic acid (9.69), Cyclobutanol (10.97), and Diethylpropylmalonate (15.63).</p> <p><strong>Conclusion: </strong>These results indicated that <em>Paecilomyces formosus</em> spores and extracted secondary bioactive metabolites could serve as promising lead organism for the development of potential novel and effective insecticidal compounds.</p> Abdulrahman Itopa Suleiman, Abba Nasidi, Rufai Nasir, Jwan’an L. Emmanuel, Nasir Sirajo Sadi, Mustapha Omenesa Idris, Abdullahi Abdulkadir Imam ##submission.copyrightStatement## Sat, 16 Oct 2021 00:00:00 +0000