Asian Journal of Biotechnology and Genetic Engineering https://journalajbge.com/index.php/AJBGE <p style="text-align: justify;"><strong>Asian Journal of Biotechnology and Genetic Engineering</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGE/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGE includes but not restricted to all aspects of Biotechnology, Genetics, Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, etc. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> Asian Journal of Biotechnology and Genetic Engineering en-US Asian Journal of Biotechnology and Genetic Engineering Bromate Causes Significant Cytotoxicity and Modulation of the Inflammatory Response https://journalajbge.com/index.php/AJBGE/article/view/30085 <p>Bromate is used as an additive in bread and also a disinfection by-product of hypochlorite treated and ozonated waters. The effect of bromate (as potassium bromate) on the proliferation of U937 cells and activation of U937-derived macrophages was investigated. First, U937 cells were incubated with bromate at different concentrations and later cell viability was assessed via the trypan blue dye staining and reduction of MTT. The production of reactive oxygen species (ROS) was also measured via the DCHF-DA assay. In further experiments, the U937 cells were transformed to the macrophage form via treatment with phorbol-12-myristate-13-acetate before incubated with different concentrations of bromate. Later the production of NO, TNF-a, and IL-6 was evaluated as indices for the activation of macrophages. The results revealed that bromate caused significant cell death when compared to control and was concentration-dependent (<em>p </em>&lt; 0.05). Bromate also significantly produced reactive oxygen species in U937 cells, and also stimulated the production of NO, TNF-a, and IL-6 in U937-derived macrophages. This indicate that bromate causes significant cytotoxicity and also trigger cells to produce significant amount of intracellular reactive oxygen species. Bromate also activated macrophages thus indicating that the cytotoxicity of bromate could involve the modulation of the inflammatory response.</p> Tebekeme Okoko ##submission.copyrightStatement## 2020-10-21 2020-10-21 1 8 Effect of Gibberellic Acid on Seed Germination and Evaluation of Agrobacterium rhizogenes Strains for the Induction of "Hairy Roots" of Phyllanthus niruri Linn. (Phyllantaceae) https://journalajbge.com/index.php/AJBGE/article/view/30086 <p><em>Phyllanthus niruri</em> L. is a medicinal plant from tropical regions with great pharmacognosic virtues scientifically proven. It is a rare herbaceous wild species in the dry season. Its use for the pharmaceutical industry requires a regular supply and it is independent of climatic hazards. The main aim to find optimal seed storage conditions with reference to the factors that limit the germination of <em>P. niruri</em> seeds and to promote the emergence of dormancy and germination of <em>P. niruri</em> seeds harvested from different agro-ecological sites in Kinshasa. The results of the present study showed high germination rates with GA3, which significantly influenced the germination of the seeds of <em>Phyllanthus niruri</em>. Seeds stored at laboratory temperature (28°C) had a good germination power than those stored at 4°C. In addition, the germination capacity of seeds stored for 7 days seems to be lower than that of those stored for 14 days. Seeds harvested at low altitude (Kisenso site) gave a higher germination rate than those harvested at high altitude (UPN and CREN-K sites).&nbsp; However, the late two sites gave almost the same germination rates.</p> <p>The <em>Agrobacterium rhizogenes</em> strains A<sub>4</sub>RSpHKN29, LBA 9402 and ATCC 15834 preserved in the Biotechnology Laboratory of CREN-K did not induce the "Hairy roots" of <em>P. niruri</em> after 27 days of culture on MS medium with sucrose but they conserved their plasmids. Nevertheless, some results obtained showed a low production of vitro-plants and transgenic roots (Hairy roots) from caulinary explants.</p> Florent Biduaya Mukeba Gédéon Ngiala Bongo Johnny Bopopi Mukoko Freddy Otono Bulubulu Myriam Mukadi Ngondo Paulin Kapepula Mutwale Odette Ngandu Kabena Koto-te-Nyiwa Ngbolua ##submission.copyrightStatement## 2020-10-31 2020-10-31 9 23