Assessment of Genetic Diversity for Cotton Leaf Curl Disease (CLCuD) and Qualitative Traits among Elite Cotton Cultivars

Syed Bilal Hussain *

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Laraib Farooq

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Zubair

Department of Forestry and Range Management, Bahauddin Zakariya University, Multan, Pakistan.

*Author to whom correspondence should be addressed.


Cotton is a major fibrous cash crop cultivated in over 80 countries because the global textile industry depends on it. In Pakistan, the primary factor reducing cotton production is cotton leaf curl disease caused by a begomovirus, a cotton leaf curl virus vectored through the whitefly, Bemisia tabaci. This disease shows various symptoms, including vein thickening, stunted plant growth, and cup-shaped outgrowth known as Enation. This study was conducted to analyze the genetic diversity for cotton leaf curl disease among 50 cotton varieties using 10 SSR primers. The results showed that 57 alleles were identified, averaging 5.7 alleles per primer. Maximum Polymorphism was exhibited by the primers NAU 2083 and NAU 2273, having PIC values of 0.8621 and 0.5874, respectively. Phylogenetic tree by neighbor-joining method showed a greater genetic diversity among the cotton genotypes under study suggesting that these cotton varieties can be utilized in future breeding programs for cotton improvement.

Keywords: Cotton, CLCuD, elite, cultivars, diversity

How to Cite

Hussain, S. B., Farooq, L., & Zubair, M. (2022). Assessment of Genetic Diversity for Cotton Leaf Curl Disease (CLCuD) and Qualitative Traits among Elite Cotton Cultivars. Asian Journal of Biotechnology and Genetic Engineering, 5(2), 220–225. Retrieved from


Download data is not yet available.


Ahmed N, Amin I, Zaidi SS-E-A, Rahman SU, Farooq M, Fauquet CM, Mansoor S. Circular DNA enrichment sequencing reveals the viral/satellites genetic diversity associated with the third epidemic of cotton leaf curl disease. Biology Methods and Protocols. 2021;6:bpab005.

Sarwar G, Nazir A, Rizwan M, Shahzadi E, Mahmood A. Assessment of genetic diversity of cotton cultivars by using correlation and principal component analysis for CLCuD tolerance, yield and quality traits. Journal of Agricultural Research. 2021;59:1-5.

Ozyigit II, Dogan I, Kaya Y, Bajrovic K, Gozukirmizi N. Cotton biotechnology: An efficient gene transfer protocol via agrobacterium tumefaciens for a greater transgenic recovery. Journal of Natural Fibers. 2022;1-15.

Sahar A, Zafar MM, Razzaq A, Manan A, Haroon M, Sajid S, Rehman A, Huijuan M, Ashraf M, Maozhi R. Genetic variability for yield and fiber related traits in genetically modified cotton. Journal of Cotton Research. 2021;4:1-10.

Azam S, Rao AQ, Shad M, Ahmed M, Gul A, Latif A, Ali MA, Husnain T, Shahid AA. Development of broad-spectrum and sustainable resistance in cotton against major insects through the combination of Bt and plant lectin genes. Plant Cell Reports. 2021;40:707-721.


Khan MaU, Shahid AA, Rao AQ, Shahid N, Latif A, Ud Din S, Husnain T. Defense strategies of cotton against whitefly transmitted CLCuV and Begomoviruses. Advancements in Life Sciences 2015;2:58-66.

Farooq T, Umar M, She X, Tang Y, He Z. Molecular phylogenetics and evolutionary analysis of a highly recombinant begomovirus, Cotton leaf curl Multan virus, and associated satellites. Virus evolution. 2021;7:veab054.

Zubair M, Zaidi SS-E-A, Shakir S, Farooq M, Amin I, Scheffler JA, Scheffler BE, Mansoor S. Multiple begomoviruses found associated with cotton leaf curl disease in Pakistan in early 1990 are back in cultivated cotton. Scientific reports. 2017;7:1-11.

Haidar KS, Khan M, Ahmad M, Sarwar N, Murtaza M, Aslam M. Evaluation of Gossypium species for resistance to cotton leaf curl Burewala virus. Annals of Applied Biology. 2010;157:135-147.

Abbas A, Iqbal MA, Rahman M-U, Paterson AH. Estimating genetic diversity among selected cotton genotypes and the identification of DNA markers associated with resistance to cotton leaf curl disease. Turkish Journal of Botany. 2015;39:1033-1041.

Çelİk S. Genetic Diversity Analysis of Some Upland Cotton (Gossypium hirsutum L.) Genotypes Using SSR Markers. Türk Doğa ve Fen Dergisi. 2022;11:80-89.

Ali I, Khan NU, Gul S, Khan SU, Bibi Z, Aslam K, Shabir G, Haq HA, Khan SA, Hussain I. Genetic diversity and population structure analysis in Upland cotton Germplasm. International Journal of Agriculture and Biology. 2019;22:669 -676.

Rehman A, Iqbal M. Genome–wide Allelic Variations and Population Structure Analysis of Cotton Genotypes using SSR Markers. Pakistan Journal of Biochemistry and Biotechnology 2022;3:91-111.