Synthesis and Biological Activities of Cu-Nanoparticles from Aspergillus niger

Syed Bilal Hussain *

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Romesa Bashir

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Sadaf Noor

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Zubair

Department of Forestry and Range Management, Bahauddin Zakariya University, Multan, Pakistan.

*Author to whom correspondence should be addressed.


Nanotechnology has received tremendous attention because its applications have expanded in a variety of fields. The biological route for the synthesis of nanoparticles become more demanding as it is eco-friendly, low cost, and not time taking procedure. In this research, Aspergillus niger filtrate is used as a reducing agent to biosynthesize copper nanoparticles (CuNPs) under controlled parameters i.e., pH, temperature, and time. Synthesized CuNPs were confirmed by UV-Visible spectroscopy and further characterized by scanning transmission electron microscopy (STEM), and Fourier transforms infrared (FTIR) spectroscopy. The UV- Visible spectroscopy exhibit a maximum peak of 540nm which confirmed the formation of CuNPs. FTIR showed two maximum peaks 3339cm-1 and 1638 cm-1. These peaks represent the presence of O-H stretching and –C=C- stretching respectively. The size ranges of CuNPs between 15nm-85nm with spherical shapes. The anti-microbial activity was tested against gram-positive and gram-negative bacteria and showed the significant antibacterial potential of CuNPs. Radical scavenging activity was confirmed by DPPH assay. The results of antioxidant activity indicated the IC50 value of CuNPs was 59.10ug/ml. Thus, CuNPs synthesized through a biological route could act as good antibacterial as well as antioxidant agents.

Keywords: Aspergillus niger, nanoparticles, copper, antibacterial, antioxidant

How to Cite

Hussain, S. B., Bashir, R., Noor, S., & Zubair, M. (2022). Synthesis and Biological Activities of Cu-Nanoparticles from Aspergillus niger. Asian Journal of Biotechnology and Genetic Engineering, 5(2), 206–219. Retrieved from


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Noor S, Shah Z, Javed A, Ali A, Hussain SB, Zafar S, et al. A fungal based synthesis method for copper nanoparticles with the determination of anticancer, antidiabetic and antibacterial activities. J Microbiol Methods. 2020;174(105966).

Naqvi STQ, Shah Z, Fatima N, Qadir MI, Ali A, Muhammad SA. Characterization and biological studies of copper nanoparticles synthesized by Aspergillus niger. J Bionanosci. 2017;11(2):136-40.

Azharuddin M, Zhu GH, Das D, Ozgur E, Uzun L, Turner AP, et al. A repertoire of biomedical applications of noble metal nanoparticles. Chem Commun. 2019; 55(49):6964-96.

Gupta C, Rabani MS, Gupta MK, Tripathi S, Pathak A. Nanoscience in Biotechnology. Apple Academic Press. 2022;241-67.

Mody VV, Siwale R, Singh A, Mody HR. Introduction to metallic nanoparticles. J Pharm Bioallied Sci. 2010;2(4):282.

Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomed: Nanotechnol Biol Med. 2010;6(2):257-62.

Iravani S, Korbekandi H, Mirmohammadi SV, Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods. Res Pharm Sci. 2014;9(6):385.

Gade A, Bonde P, Ingle A, Marcato P, Duran N, Rai M. Exploitation of Aspergillus niger for synthesis of silver nanoparticles. J Biobased Mater Bioenergy. 2008;2(3): 243-47.

Farrag HMM, Mostafa FaaM, Mohamed ME, Huseein EaM. Green biosynthesis of silver nanoparticles by Aspergillus niger and its antiamoebic effect against Allovahlkampfia spelaea trophozoite and cyst. Experimen Parasit. 2020; 219(108031.

Sharma M, Sharma A, Majumder S. Synthesis, microbial susceptibility and anti-cancerous properties of copper oxide nanoparticles-review. Nano Express. 2020;1(1):012003.

Suood AM, Saleh MK, Thalij KM. Synthesis of Copper Nanoparticles Using Aspergillus Niger and Their Efficacy Against Pathogenic Staphylococcus Aureus. in IOP Conference Series: Earth and Environmental Science. IOP Publishing; 2021.

Jain N, Bhargava A, Majumdar S, Tarafdar J, Panwar J. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus flavus NJP08: a mechanism perspective. Nanoscale. 2011; 3(2):635-41.

Afzal H, Shazad S, Qamar S, Nisa S. Morphological identification of Aspergillus species from the soil of Larkana District (Sindh, Pakistan). Asian J Agric Sci. 2013;1(105):e17.

Mcclenny N. Laboratory detection and identification of Aspergillus species by microscopic observation and culture: the traditional approach. Med Myco. 2005; 43(sup1):125-28.

Dashen M, Ado SA, Ameh J, Amapu T, Zakari H. Screening and improvement of local isolates of Aspergillus niger for citric acid production. Bayero J Pure Appl Sci. 2013;6(1):105-11.

Schwarz P, Bretagne S, Gantier J-C, Garcia-Hermoso D, Lortholary O, Dromer F, et al. Molecular identification of zygomycetes from culture and experimentally infected tissues. J Clin Microbiol. 2006;44(2):340-49.

Zhang Y, Zhang S, Liu X, Wen H, Wang M. A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Letters in applied microbiology. 2010;51(1):114-18.

Embong Z, Hitam WHW, Yean CY, Rashid NHA, Kamarudin B, Abidin SKZ, et al. Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis. BMC Ophthal. 2008;8(1):7.

Felsenstein J. Comparative methods with sampling error and within-species variation: contrasts revisited and revised. Am Nat. 2008;171(6):713-25.

Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-25.

Abd El-Aziz AR, Al-Othman MR, Alsohaibani SA, Mahmoud MA, Rushdy S. Extracellular biosynthesis and characterization of silver nanoparticles using Aspergillus niger isolated from Saudi Arabia (Strain KSU-12). Digest J Nanomat Biostruct. 2012;7(1491-99.

Saitawadekar A, Kakde UB. Green Synthesis of Copper Nanoparticles Using Aspergillus Flavus. J Crit Rev. 2020;7(10):83-90.

Das PE, Abu-Yousef IA, Majdalawieh AF, Narasimhan S, Poltronieri P. Green synthesis of encapsulated copper nanoparticles using a hydroalcoholic extract of Moringa oleifera leaves and assessment of their antioxidant and antimicrobial activities. Molecules. 2020; 25(3):555.

Michen B, Geers C, Vanhecke D, Endes C, Rothen-Rutishauser B, Balog S, et al. Avoiding drying-artifacts in transmission electron microscopy: Characterizing the size and colloidal state of nanoparticles. Sci Rep. 2015;5(1):1-7.

Subbaiya R, Selvam MM. Green Synthesis of Copper Nanoparticles from Hibicus Rosasinensis and their antimicrobial, antioxidant activities. Res J Pharm Biol. 2015;6(2):1183-90.

Rajeshkumar S, Menon S, Kumar SV, Tambuwala MM, Bakshi HA, Mehta M, et al. Antibacterial and antioxidant potential of biosynthesized copper nanoparticles mediated through Cissus arnotiana plant extract. J Photochem Photobiol B:Biol. 2019;197(111531.

Li G, He D, Qian Y, Guan B, Gao S, Cui Y, et al. Fungus-mediated green synthesis of silver nanoparticles using Aspergillus terreus. International Journal of Molecular Sciences. 2011;13(1):466-76.

Din MI, Rehan R. Synthesis, characterization, and applications of copper nanoparticles. Anal Lett. 2017; 50(1):50-62.

Salvadori MR, Ando RA, Oller Do Nascimento CA, Correa B. Bioremediation from wastewater and extracellular synthesis of copper nanoparticles by the fungus Trichoderma koningiopsis. J Environ Sci Health A. 2014;49(11): 1286-95.

Yadav A, Kon K, Kratosova G, Duran N, Ingle AP, Rai M. Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research. Biotechnol Lett. 2015;37(11): 2099-120.

Amer M, Awwad A. Green synthesis of copper nanoparticles by Citrus limon fruits extract, characterization and antibacterial activity. Chem Internation. 2020;7(1):1-8.

Kiranmai M, Kadimcharla K, Keesara NR, Fatima SN, Bommena P, Batchu UR. Green synthesis of stable copper nanoparticles and synergistic activity with antibiotics. Indian J Pharm Sci. 2017;79(5): 695-700.