Physico-Chemical Analysis and Aerobic Bioremediation of Paint Effluent Using Pseudomonas aeruginosa and Bacillus subtilis
Asian Journal of Biotechnology and Genetic Engineering,
Page 45-52
Abstract
Bioremediation uses biological techniques to reduce the harmful effects of pollutants in the environment, including wastewater. It is a handy tool for various applications in environmental protection. In developing strategies for bioremediation of paint effluent, untreated paint effluent from Dulux Plc was inoculated with two aerobic microorganisms, Pseudomonas aeruginosa and Bacillus subtilis. Microbiological and physicochemical analysis was carried out for five weeks upon inoculation of effluent with Pseudomonas aeruginosa P, Bacillus subtilis B, in separate setups, effluent sample inoculated with both organisms M, to observe their synergy and effluent sample without inoculation, served as a control sample to compare the abilities of the native effluent microflora with that of the introduced microorganisms. The cultures of microorganisms were observed in nutrient broth until the exponential phase before their inoculation into the effluent sample. The bacterial cultures of Pseudomonas aeruginosa, Bacillus subtilis and consortium contained 4.2 x 108cfu/ml, 3.8 x 109cfu/ml and 5.2 x 109cfu/ml, respectively, when inoculated into the experimental setups P, B and M, in the experimental samples, BOD and all the inorganic nutrient sources tested decreased rapidly with a proportional increase in the population densities. This trend was firm for PO43- and NO3, which eventually became limiting in the experimental and control samples. Inoculating microorganisms with the addition of appropriate inorganic nutrients may be a suitable method for rapid bioremediation of paint effluent.
Keywords:
- Bacterial
- bioremediation
- paint effluent
- waste water management
- physicochemical analysis
How to Cite
References
Mahdavi TA, Donnelly T, Andersonm GK. Colourremovalfrom simulated dye wastewater using a two-phase anaerobic packed bedreactor, Water Res. 2001;35: 425–432.
Ojoawo SO, Udayakumar G, Naik P. Phytoremediation of phosphorus and nitrogen with canna x generalis reeds in domestic wastewater through NMAMIT constructed wetland. Aquatic Procedia. 2015;4:349–356.
Worku A, Tefera N, Kloos H, et al. Biosremediation of brewery wastewater using hydroponics planted with vetiver grass in Addis Ababa, Ethiopia. Bioresour. Bioprocess. 2018;5:39-43.
Sasikumar CS, Papinazath T. Environmental management: Bioremediation of Environment" Martin, J., Bunch, V. eds. Proceedings of the third international conference on environment and health, Chennai, India. 2003;465-469.
Melcalf L, Eddy HR. Wastewater engineering, treatment and reuse (4th edition), Tale McGraw-Hill, New York; 2003.
Ram SL, Pravin US, Deepali SP. Study on Physico-chemical Parameters of Waste Water Effluents from Taloja Industrial Area of Mumbai, India. Int. J. Ecosyst. 2011;1: 1–9.
Aboulhassan MA, Souabi S, Yaacoubi A, Baudu M. Treatment of Paint Manufacturing Wastewater the Combination of Chemical and Biological Processes. Inter. J. Sci. Environ. Technol. 2014;3:1747–1758.
Nwachukwu SCU, Akpata TVI. Principles of Quantitative Microbiology; 2003.
Central California Area Office- American River Water Education Center. Water facts- worldwide water supply. Bureau of reclamation; 2020.
Usharani K, Umarani K, Ayyasamy PM, Shanthi K, Lakshmanaperumalsamy P. Physico-chemical and bacteriological characteristics of Noyyal River and groundwater quality of Perur, India. J. Appl. Sci. Environ. Managccc. 2010;14:29–35.
Endeshaw A, Birhanu G, Zerihun T, Misganaw W. The role of microorganisms in bioremediation- A review. Open Journal of Environmental Biology. 2017;7.20-25.
Tolulope E, Aniyikaiye T, Oluseyi J, Odiyo O, Joshua NE. Physico-Chemical Analysis of Wastewater Discharge from Selected Paint Industries in Lagos, Nigeria. Intl. J.Envir. Res. Public Health. 2019;16:123-127.
Rachna B, Disha J. Water Quality Assessment of Lake Water: A Review. Sustain. WaterResour. Manag. 2016;2: 161–173.
-
Abstract View: 8 times
PDF Download: 1 times
