Evaluating the Assault Caused by Nauclea latifolia on the Biochemical Parameters of Wistar Rats Following Testicular Tissue Damage
Asian Journal of Biotechnology and Genetic Engineering,
Background: The use of herbal products is very common among rural dwellers, although these products posses prophylactic, therapeutic and curative capacities. Unguarded doses most times often result in cellular and tissue damage in the body. This study was undertaken to evaluate the assault caused by Nauclea latifolia on lactate dehydrogenase, superoxide dismutase and DNA damage assay in Wistar rats following testicular tissue damage.
Methods: Forty (40) male Wistar rats divided into eight (8) groups were used for this investigation (n=5). The animals were administered with 3mg of alcohol/kg b.w alone, 5 mg of testosterone/kg b.w, 500 mg of ethanolic leaf extract of Nauclea latifolia/kg b.w, 1000 mg of ethanolic leaf extract of Nauclea latifolia/kg b.w, 1500 mg of ethanolic leaf extract of Nauclea latifolia/kg b.w, 3 mg of alcohol/kg b.w and 500 mg of of ethanolic leaf extract of Nauclea latifolia/kg b.w, 5mg of testosterone and 500 mg of ethanolic leaf extract of Nauclea latifolia/kg b.w and the control received 15 % tween 80 (10 ml of tween 80/kg b.w ) respectively. The experiment lasted for 21 days.
Results: Ethanolic leaf extract of Nauclea latifolia at doses of 500, 1000 and 1500 mg/kg b.w of rats significantly increased DNA damage and lactate dehydrogenase.
Conclusion: Increased in these biochemical parameters signify increased tissue damage.
- Nauclea latifolia
- lactate dehydrogenase
- superoxide dismutase
- DNA damage assay
- tissue damage
How to Cite
Gidday M, Asfaw Z, Almqvist T. and Woldu Z. An Ethnobotanical study of medicinal plants used by the zay people in Ethiopia. Journal of Ethnopharmacology. 2003;85: 43-52.
World Health Organization. The Promotion and development of traditional medicine. geneva: Technical Report Series. 2002; 622.
Sofowora A. Medicinal Plants and Traditional Medicine in Africa, 2nd edition, Ibadan, Nigeria: Spectrum Books Ltd. 1993;130.
Chopra RN, Nayar SL. and Chopra IC. Glossary of India medicinal plants: council of scientific and India research, New Delhi Indian; 1980.
Edem GD, Okon KA, Essien SI. and Bassey EI. Lantana camara: A potent influential factor in improving the gastric mucosa of wistar rats ravaged by ulcer. Biological and Clinical Sciences Research Journal. 2021;1:1-4.
Iwu M. Handbook of African Medicinal Plants, 2nd edition, Boca Raton, FL: CRC Press. 1993;28-31.
Deeni Y. and Hussain H. Screening for Anti-microbial activity and alkaloids of nauclea latifolia. Journal of Ethnopharmacology. 1991;35:91-96.
American Physiological Society. Guiding principles for research involving animals and human beings. American Journal of Physiological Regulatory Comprehensive Physiology, 2002;283:281-283.
Munoz IM, Jowsy PA, Toth R. and Rouse J. Phospho-epitope Binding by BRCT Domains of HPTIP controls multiple aspects of the response to DNA damage. Nucleic Acids Research. 2007;35:5312–5322.
Sasaki H. and Matsui Y. Epigenetic events in mammalian germ-cell development reprogramming and beyond. Nature Review Genetics. 2008;9:129-140.
Munoz IM. and Rouse J.Control of histone methylation and genome stability by PTIP. Reproduction, 2009;10(23):239-245.
Clausen J. and Ovlisen B. Lactate dehydrogenase iso-enzymes of human semen. Journal of Biochemistry. 1965;97: 513-517.
Donatus EO. and Nnamdi FU. Exotic multifaceted medicinal plants of drugs and pharmaceutical industries. African Journal of Biotechnology. 2009;8(25):7271- 7282.
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