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Several species of shellfish from the Bivalve class have unique adaptability to attach their self to a substrate as an effort of defense in strong currents. This is possible by the presence of a special protein which able to coordinate strongly with the substrate even underwater, called Mussel Foot Protein (MFP). MFP is a protein composed of 85 decapeptides where the tyrosine residue group undergoes a process to post-translational become 3,4-dihydroxyphenilalanine or DOPA. The development of research that has been carried out has successfully used MFP in various fields including cell and tissue culture. The use of MFP as a matrix in the culture of several cells and tissues showed an increase in cell quality, cell adhesion and cell growth rate. The existence of several optimizations such as adjustment to optimum environmental conditions and the addition of other additives that support the culture process have also been reported. This article will discuss the latest developments related to MFP application in cell and tissue culture along with the optimization efforts that have been reported. Opportunities for future research development related to MFP applications, especially in the field of biotechnology, will also be discussed accordingly.
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