Genetic Profiling of Cynodon dactylon Species Using ISSR Markers and Its Pharmacological Activities

Main Article Content

Chandrika S. Tanthry
Saswati Mandal
S. D. Shruthi


Aim: Cynodon dactylon is a grass variety of the tropical and sub-tropical region belonging to the family Poaceae. In this study genetic profiling of 3 different varieties of Cynodon dactylon were done using ISSR primers.

Methodology: Polymorphism patterns were observed by amplifying DNA with 8 sets of primers such as OPA16, OPB11, OPC06 etc. PyElph tool was used to understand the genetic similarity of the samples and accordingly the relatedness was shown by dendrogram construction by DendroUPGMA. The grass samples were also used to check their antibacterial activity against potential pathogenic bacteria like Pseudomonas aeruginosa, Salmonella typhi, Chrysobacterium gleum etc. The plant species had showed a very satisfactory inhibition against the bacteria. In vitro anti-diabetic activity of Cynodon dactylon was checked by calculating its ability to inhibit the enzyme alpha-amylase and the anti-inflammatory activity was carried out by calculating the inhibition percentage of albumin degradation.

Outcome: All these experiments were done to study the pharmacological property as well as to gather knowledge on the genetic make-up of Cynodon dactylon. Overall, the final outcome supports the ethanobotanical claim of this plant.

Anti-diabetic, anti-inflammatory, Bermuda grass, ISSR primers, dendrogram, polymorphism

Article Details

How to Cite
Tanthry, C. S., Mandal, S., & Shruthi, S. D. (2020). Genetic Profiling of Cynodon dactylon Species Using ISSR Markers and Its Pharmacological Activities. Asian Journal of Biotechnology and Genetic Engineering, 3(3), 1-10. Retrieved from
Original Research Article

References Bermuda Grass (Cynodon dactylon); 2020.

Tropical.the Cynodon dactylon - Useful Tropical Plants; 2020.

Dong M and de Kroon H. Plasticity in Morphology and Biomass Allocation in Cynodon dactylon, a Grass Species Forming Stolons and Rhizomes. Oikos. 1994;70(1):99.

Dong M. Morphology and growth of stolons and rhizomes in three clonal grasses, as affected by different light supply. Springer Link; 1995. Cynodon dactylon (Bermuda Grass); 2020.

Singh S, Kesari A, Gupta R, Jaiswal D, Watal G. Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. Journal of Ethnopharmacology. 2007;114(2):174-179.

Al-Snafi P. Chemical constituents and pharmacological effects of Cynodon dactylon- A Review. IOSR Journal of Pharmacy (IOSRPHR). 2016;06(07):17-31.

Farsani T, Etemadi N, Sayed-Tabatabaei B, Talebi M. Assessment of Genetic Diversity of Bermuda grass (Cynodon dactylon) using ISSR Markers. International Journal of Molecular Sciences. 2011;13(1):383-392.

Ashokkumar K. Cynodon dactylon (L.)Pers. An updated review of its phytochemistry and pharmacology. Journal of Medicinal Plants Research. 2013;7:7.

Nagori B, Solanki R. Cynodon dactylon (L.) Pers.: A Valuable Medicinal Plant. Research Journal of Medicinal Plant. 2011;5(5):508-514.

Langfelder P, Zhang B, Horvath S. Defining clusters from a hierarchical cluster tree: the Dynamic Tree Cut package for R. Bioinformatics. 2007;24(5):719-720.

Vallvé S, Puigbò P. DendroUPGMA: A dendrogramconstruction utility [Ebook]. 2009;14.


Sabitha V, Panneerselvam K, Ramachandran S. In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects in aqueous extracts of Abelmoscus esculentus(L.) Moench. Asian Pac J Trop Biomed. 2012;2(2):1624.

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences. 2010;2(1):146-155.

Al-Marzoqi A, Al Taee Z. Pseudomonas aeruginosa: Antibiotic resistance pattern to different isolates in Al-Hillah city, Iraq [Ebook] (3rd ed., p. 7). Journal of Natural Sciences Research; 2013.

Xin X, Kvitko B, He S. Pseudomonas syringae: what it takes to be a pathogen. Nature Reviews Microbiology. 2018;16(5):316-328.

Baby TGR, Das SP, RKG. Chryseobacterium Gleum Urinary Tract Infection. Genes Review. 2015;1(1):1-5.

Easow J, Rajendran P, Muthusamy S, Balaji V, Rakesh G. Urinary tract infection due to Chryseobacterium gleum, an uncommon pathogen. Indian Journal of Pathology and Microbiology. 2016;59(4):551.

Abdalhamid B, Elhadi N, Alsamman K, Aljindan R. Chryseobacterium gleum pneumonia in an infant with nephrotic syndrome. Idcases. 2016;5:34-36.

Garg V, Paliwal S. Anti-Inflammatory Activity of Aqueous Extract of Cynodon dactylon. International Journal of Pharmacology. 2011;7(3):370-375.

Ramya S, Vijayanand N, Rathinavel S. Antidiabetic activity of Cynodon dactylon (l.) Pers. Extracts in alloxan-induced RATS [Ebook]. 2014;6:5.

Suresh Kumarasamy P, Deepa R, Harisaranraj V, Vaira Achudhan. Antimicrobial and Phytochemical Investigation of the Leaves of Carica papaya L., Cynodon dactylon(L.) Pers., Euphorbia hirta L., Melia azedarach L. and Psidium guajava L. [Ebook]. 2008;12:8.

Khodabakhshzadeh R, Mohamadabadi MR, Esmailizadeh AK, Moradi Shahrebabak H, Bordbar F, et al. Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Polish Journal of Veterinary Sciences. 2016;19:281-289.

Askari N, Mohammadabadi MR, Baghizadeh A. ISSR markers for assessing DNA polymorphism and genetic characterization of cattle, goat and sheep populations. Iranian Journal of Biotechnology. 2011;9:222-229.

Shojaei M, Mohammad Abadi MR, Asadi Fozi M, Dayani O, Khezri A, et al. Association of growth trait and Leptin gene polymorphism in Kermani sheep. Journal of Cell and Molecular Research. 2010;2:67-73.

Mousavizadeh A, Mohammad Abadi MR, Torabi A, Nassiry MR, Ghiasi H, et al. Genetic polymorphism at the growth hormone locus in Iranian Talli goats by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Iranian Journal of Biotechnology. 2009;7:51-53.

Zamani P, Akhondi M, Mohammadabadi MR, Saki AA, Ershadi A, et al. Genetic variation of Mehraban sheep using two inter simple sequence repeat (ISSR) markers. African Journal of Biotechnology. 2011;10:1812-1817.

Mohammadabadi MR, Esfandyarpoor E, Mousapour A. Using Inter Simple Sequence Repeat Multi-Loci Markers for Studying Genetic Diversity in Kermani Sheep. Journal of Research and Development. 2017;5(2):154

Ghasemi M, Baghizadeh A, Mohammadabadi MR. Determination of genetic polymorphism in Kerman Holstein and Jersey cattle population using ISSR markers. Australian Journal of Basic Applied Science. 2010;4:5758-5760.

Zhigileva ON, Baranova OG, Pozhidaev VV, Brol IS, Moiseenko TI. Comparative analysis of using isozyme and ISSR-PCR markers for population differentiation of Cyprinid fish. Turkish Journal of Fishery and Aquatic Science. 2013;13:159-168.

Damodar Reddy K, Nagaraju J, Abraham EG. Genetic characterization of the silkworm Bombyx mori by simple sequence repeat (SSR)-anchored PCR. Heredity. 1999;83:681-687.

Renny M, Julio NB, Bernardi SF, Gardenal CN, Oyarzabal MI. Estimation of genetic variability level in inbred CF1 mouse lines selected for body weight. Journal of Genetics. 2014;93:483-487.