Evaluation of the Growth of G6 Transgenic Mutiara Catfish (Clarias gariepinus) after Mixed Feeding of Snail Flour (Achatina fulica) and Commercial Feed
Published: 2024-07-13
Page: 173-184
Issue: 2024 - Volume 7 [Issue 2]
Ibnu D. Buwono *
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia.
Roffi Grandiosa. H
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia.
Iskandar
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia.
Putri Budi. S
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
This research was conducted in Hatchery Building 4, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The purpose of this experiment was to obtain the optimal ratio of snail flour mixture and commercial feed (Hi Pro Vite 781) to support growth, feed efficiency ratio, protein retention value, and carbohydrate retention in G6 transgenic mutiara catfish. The study was carried out experimentally with four treatments and three replicates (CRD), the treatment given was the ratio of commercial feed mixture: snail flour, namely A (60%: 40%), B (40%: 60%), C (20%: 80%) for G6 transgenic mutiara catfish and A* treatment (60%: 40%) for non-transgenic catfish (sangkuriang catfish as a control). Significant differences between treatments were tested using the Duncan Multiple Range Test (DMRT) with a confidence level of 95%. Data on absolute weight growth (Wg), feed conversion ratio (FCR), protein efficiency ratio (PER), protein retention, and carbohydrate retention were analyzed using Sigmaplot 15.0. The results showed that treatment B using 40% snail flour showed the highest body weight gain (402.357 g), lowest FCR (0.69), protein efficiency ratio (16.91%), best protein retention (53.01%) and lowest carbohydrate retention (11.32%) compared to other treatments. The best results in treatment B were due to Transgenesis-GH inducing a protein sparing effect, showing that the efficiency of feed use and protein use in transgenic G6 mutiara catfish increased the conversion of mixed feed carbohydrates of treatment B into protein. As a consequence, the growth of transgenic catfish is increased higher than that of non-transgenic fish, which is indicated by increased retention value and protein efficiency.
Keywords: G6 mutiara ransgenic catfish, snail meal and commercial feed, protein retention and efficiency, carbohydrate retention, absolute growth
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References
Karimah, U, Samidjan I. Performa pertumbuhan dan kelulushidupan ikan nila gift (Oreochromis niloticus) yang diberi jumlah pakan yang berbeda. Journal of Aquaculture Management and Technology. 2018;7(1):128-135.
Pathmasothy S. A review of feed, their processing and feeding techniques in Malaysian aquaculture system. In: Proceedings of the International Conference on Development and Management of Dopical Living Aquatic Resources (ed. by H.H. Chan KJ, Ang HM, Ibrahim Ambak MA). Universiti Pertanian Malaysia. 1983;126-131.
Halver JE. Fish Nutrition. Second Edition. Academic Press Inc. California; 1989.
Pillay TVR, Kutty MN. Aquaculture: Principles and practices. Second edition. USA: Blackwell publishing Ltd; 2005.
Mansyur A, Kamaruddin. Analisis bahan dan manfaatnya dalam menyusun formulasi pakan ikan budidaya. Media Akuakultur. 2008;1(3):113-117.
Mudjiman, A. Makanan Ikan Edisi Revisi, Penebar Swadaya. Depok. 2004.
Rosalia D, Yudha IG, Santoso L. Kajian Pemanfaatan Tepung Bekicot (Achatina fulica) Sebagai Bahan Baku Pakan Benih Ikan Gabus Channa striata (Bloch, 1793). Biospecies. 2018;11(1).
Buwono ID, Grandiosa R, Mulyani Y, Pratiwy FM. GnRHr, LHr and Vg gene expression levels and ovarian development of G5 transgenic mutiara female catfish (Clarias gariepinus) after exposure photoperiod induction. Discover Applied Sciences. 2024;6(2): 63.
Leggatt RA, Raven PA, Mommsen TP, Sakhrani D, Higgs D, Devlin RH. Growth hormone transgenesis influences carbohydrate, lipid and protein metabolism capacity for energy production in coho salmon (Oncorhynchus kisutch). Comp Biochem Physiol Part B. 2009;154: 121–133.
Buwono ID, Iskandar I, dan R. Grandiosa. growth hormone transgenesis and feed composition influence growth and protein amino acid content in transgenic G3 mutiara catfish (Clarias gariepinus). Aquaculture Internatiomal. 2021;29:431-451.
Hidayat D,Sasanti AD. Kelangsungan hidup, pertumbuhan dan efisiensi pakan ikan gabus (channa striata)yang diberi pakan berbahan baku tepung keong mas (Pomacea sp).Jurnal akuakultur rawa Indonesia. 2013;1(2):161-172.
Djarijah AS. Natural Feed. Kanisius, Yogyakarta. p. 1995;87.
Zeitoun IH., Ullrey DE, Mages WT. Quantifying nutrient requirement of fish. J. Fish Res. Board of Canada. 1976;33:167-172.
Takeuchi T. Laboratory work chemical evaluation of dietary nutrients.p.. In: Watanabe, T. (Ed). Fish nutrition and mariculture JICA. The general aquaculture course. Kanagawa international fisheries training centre. Japan International Cooperation Agency (JICA). Tokyo. 1988;23:179-233.
Dewi RRSPS, dan Tahapari E. Pemanfaatan probiotik komersial pada pembesaran ikan lele (Clarias gariepinus). Jurnal Riset Akuakultur. 2017;12 (3):275–281.
AOAC. The official methods of analyses 15th edn. Association of official analytical chemists international. Arlington, Virginia, USA; 1990.
Sogbesan AO, Ugwumba AAA. Nutritional values of some non-conventional animal protein feedstuffs usedas fishmeal supplement in aquaculture practices in Nigeria.University of Ibadan. Ibadan. Nigeria. 2008;8:159-164.
Bureau DP, Cho CY. An Introduction to Nutrition and Feeding of Fish. Fish Nutrition Research Laboratory Departement of Animal and Poultry Science. University of Guelph. Ontario. Ca nada. 1999;36 hal.
Raven PA, Devlin RH, Higgs DA. Infuence of dietary digestible energy content on growth, protein and energy utilization and body composition of growth hormone transgenic and non-transgenic coho salmon (Oncorhynchus kisutch). Aquaculture. 2006;254:730–747.
Dong GF, Yang YO, Song XM, Yu L, Zhao TT, Huang GL, Zhang JL. Comparative effects of dietary supplementation with maggot meal and soybean meal in gibel carp (Carassius auratus gibelio) and darkbarbel catfish (Pelteobagrus vachelli): growth performance and antioxidant responses. Aquaculture Nutrition. 2013; 19(4):543-554.
Sari MP, Helmizuryani S. Adji dan K. Khotimah. Pengaruh Interval Pemuasaan Pakan terhadap Pertumbuhan dan Kelangsungan Hidup Ikan Patin (Pangasius hypopthalmus). Journal of Global Sustainable Agriculture. 2022;2(1): 36-43.
Raven PA, Uh M, Sakhrani D, Beckman BR, Cooper K, Pinter J, Leder EH, Silverstein J, Devlin RH. Endocrine effects of growth hormone over expression in transgenic Coho salmon. General and Comparative Endocrinology. 2008;159:26-37.
Devlin RH, D'Andrade M, Mitchell U, Carlo AB. Population effects of growth hormone transgenic coho salmon depend on food availability and genotype by environment interactions. PNAS. 2004;25:9303 – 9308.
Cook JT, McNiven MA, Richardson GF, Sutterlin AM. Gowth Rate, Body Composition, and Feed Digestibility/ Conversion of Gowth Enhanced Atlantic Salmon (Salmo salar). Aquaculture. 2000;188:15 – 32.
Zhong CY, Song Y, Wang Y, Li Y, Liao L, Xie S, Zhu Z, Hu W. Growth hormone transgene effects on growth performance are inconsistent among offspring derived from different homozygous transgenic common carp (Cyprinus carpio L.). Aquaculture. 2012;356-357:404-41.
Melzer K. Carbohydrate and fat utilization during rest and physical activity. European e-Journal of Clinical Nutrition and Metabolism. 2011;6:45 –52.
Okanlawon SS, Oladipupo SA. Nutritional evaluation of snail offal meal as animal protein supplement in the diets of Clarias gariepinus (Burchell, 1822) fingerlings. World Journal of Fish and Marine Sciences. 2010;2(2):103-108.
Pa ARB, Rebhung F, Lukas AYH. Pengaruh pemanbahan tepung daging bekicot (Achatina fulica) dalam pakan terhadap pertumbuhan dan kelulushidupan ikan bandeng (Chanos chanos, Forskall). Jurnal Aquatik. 2020;3(1):59-71.
Haghparast P, Falahatkar B, Khoshkholgh MR, Meknatkhah B. Influence of dietary protein/lipid ratio on growth performance and body composition of Aspikutum, a new hybrid of Leuciscus aspius ♀ × Rutilus frisii ♂ (Teleostei: Cyprinidae). Ichthyol. 2016;3 (4):304–315.
Suprayudi MA, Setiawati M, Mokoginta I. Pengaruh rasio protein energi yang berbeda terhadap pertumbuhan ikan gurami Osphronemus goramy L. Skripsi. Bogor (ID): Institut Pertanian Bogor; 2004.
Yigit, M., Yardim, Ö., & Koshio, S. The protein sparing effects of high lipid levels in diets for rainbow trout (Oncorhynchus mykiss, W. 1792) with special reference to reduction of total nitrogen excretion. Israeli Journal of Aquaculture-Bamidgeh. 2002; 54.
Viola S, dan rappaport. The extra caloric effect of oil in the nutrition of carp. Bamigdeh. 1979;31(3):51-69.
Khordi KMGH. Delicious taste, delicious fortunately - smart fish farming in ponds intensively. Lily Publisher. Yogyakarta; 2010.
Li X, Zheng S, Wu G. Nutrition and functions of amino acids in fish. Amino acids in nutrition and health: Amino acids in the nutrition of companion, zoo and farm animals. 2021;133-168.
Buwono ID, AHS A. Growth Performance in sangkuriang catfish (Clarias gariepinus Var. Sangkuriang) and transgenic mutiara catfish (Clarias gariepinus) using low protein feed. Asian Journal of Biotechnology and Bioresource Technology. 2023;9(3):10-19.
Buwono ID, Junianto J, Iskandar I, Alimuddin A. Gowth and expression level of gowth hormone in transgenic mutiara catfish second generation. Journal of Biotech Research. 2019;10 :102-109.
Laksana DP, Subaidah S, Junior MZ, Alimuddin, O. Carman. Pertumbuhan Pascalarva Udang Vaname yang Diberi Larutan Hormon Pertumbuhan Rekombinan. Jurnal Akuakultur Indonesia. 2013; 20 (2):95-100.
Indonesian National Standard (SNI). Dumbo Catfish (Clarias sp). National Standardization Agency. Jakarta. 2014; SNI 6484.3.
Rachmawati D, Samidjan I, Soedarto J P and Reksosari V 2015 PENA Aquatics 12 24–32[10].
Diana JS, Szyper JP, Batterson TR, Boyd CE, Piedrahita RH. Water quality in ponds. Dynamics of pond aquaculture. 2017; 14:53-71.