Asian Journal of Biotechnology and Genetic Engineering

Antibacterial Activity of Vitamin c and Sweet Orange Juice (Citrus sinensis) Extract Against Staphylococcus aureus Isolated from Wound Infection among Patient Attending Bodinga General Hospital, Sokoto, Nigeria

Ibrahim, Jamilu *

Department of Biological Sciences, Sokoto State University, Sokoto, Nigeria.

Sani, Yahaya Lema

Department of Biological Sciences, Sokoto State University, Sokoto, Nigeria.

*Author to whom correspondence should be addressed.

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Abstract

Staphylococcus aureus is a major human pathogen responsible for a wide range of clinical infections, including bacteremia, infective endocarditis, osteoarticular infections, skin and soft tissue infections, pleuropulmonary infections, and device-related infections. This study aimed to evaluate the antibacterial activity of Vitamin C and Sweet Orange Juice (Citrus sinensis) extract against Staphylococcus aureus isolated from wound infections in patients at Bodinga General Hospital, Sokoto. Ten samples were collected and analyzed using Gram staining and standard biochemical tests. The results indicated that both Vitamin C and Sweet Orange Juice exhibited the highest zones of inhibition at a 100% concentration, followed by 70%, 50%, and the lowest at 25% concentration. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for both Vitamin C and Sweet Orange Juice were determined to be 50% and 70%, respectively. Maintaining good personal hygiene and environmental sanitation can help reduce the infection rates in the study area, as well as in Nigeria and globally.

Keywords: Antibacterial activity, juice extract, human pathogen, gram staining, antimicrobial properties, antibiotic resistance

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