Use of CRISPR- CAS to Save the Climate Affected Rice: A Review

Muaz Bin Khalid *

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Muhammad Haris

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Muhammad Arslan

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Adnan Shoukat

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Muhammad Faizan

Department of Biochemistry, University of Agriculture Faisalabad, Pakistan.


Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Eza Fatima

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Zubair Zaheer

Institute of Horticultural Sciences, University of Agriculture Faisalabad, Pakistan.

Ali Hassan

Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

Usama Waheed

Department of Plant breeding and genetics, University of Agriculture Faisalabad, Pakistan.

*Author to whom correspondence should be addressed.


The productivity of agriculture has been greatly impacted by climate change, especially rice production, several biotic and abiotic stressors, including heat, salt, drought, heavy metals, rice blast, and bacterial blight, can severely reduce rice yields and jeopardize global food security. Numerous strategies have been employed in this regard to cultivate rice varieties that may be able to adapt to changing climate conditions. Nowadays, crop development has undergone a revolution thanks to gene editing (GE) technologies. As one of the most useful, dependable, affordable, and labor-efficient GE technologies available, the CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein) system has gained favor among plant researchers, notably rice breeders and geneticists. Since 2013 (the year the technique was first deployed in rice), CRISPR/Cas-based GE technologies have been employed to develop a variety of trait-specific climate-resilient rice lines. Several studies that have already been released attest to the effective use of GE technologies for rice development. However, this review's primary objective is to provide a succinct, thoroughly researched summary of the most current studies (starting in 2020) on the application of GE tools—particularly CRISPR-based systems for the production of CRISPR rice—to address the serious global climate change dilemma.

Keywords: Climate resilience, Oryza sativa L., gene editing, CRISPR-Cas system, food security

How to Cite

Khalid , Muaz Bin, Muhammad Haris, Muhammad Arslan, Adnan Shoukat, Muhammad Faizan, Amina, Eza Fatima, Zubair Zaheer, Ali Hassan, and Usama Waheed. 2023. “Use of CRISPR- CAS to Save the Climate Affected Rice: A Review”. Asian Journal of Biotechnology and Genetic Engineering 6 (2):179-90.


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