Asian Journal of Biotechnology and Genetic Engineering

Identification of Molecular Diversity in Gossypium hirsutum L. Against Whitefly Resistance Using SSR Markers

Syed Bilal Hussain

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Tayyab *

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Adnan Shah Bukhari

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.

Muhammad Zubair

Department of Forestry and Range Management, FAS and T, Bahauddin Zakariya University, Multan, Pakistan.

*Author to whom correspondence should be addressed.

---

Abstract

Whitefly is a major factor in reducing cotton yield worldwide more specifically in Asian Countries So to deal with this whitefly there is a need to separate genetically diverse varieties that can combat it due to its unique genetic source. In this study, genetic diversity analysis of cotton cultivars was done against whitefly resistance using simple sequence repeat (SSR) markers. For this, 75 cultivars of cotton were used to analyze genetic diversity by using 25 SSR markers out of which 9 markers were polymorphic. These markers amplified a total of 46 alleles with an average of 5.11 alleles per marker. These varieties showed a high value of gene diversity that ranged from 0.624 to 0.877 with a mean value of 0.735. Polymorphism information content (PIC) for selected genotypes ranged from 0.562 to 0.867 with a mean value of 0.704. Based on these 9 polymorphic markers 75 selected genotypes were divided into 3 major groups in a phylogenetic tree. The elected cultivars of cotton were highly polymorphic and could be used for further breeding to improve cotton accessions.

Keywords: Whitefly, CLCuD, gene diversity, CCRI, SSR, PIC

---

---

---

References

Lee JA and Fang DD. Cotton as a world crop: origin, history, and current status. Cotton. 2015; 57:1-23.

DOI: 10.2134/agronmonogr57.2013.001

Munir H, Rasul F, Ahmad A, Sajid M, Ayub S, Arif M, et al. Diverse uses of cotton: From products to byproducts. Cotton Production and Uses: Agronomy, Crop Protection, and Postharvest Technologies. 2020: 629-641.

Imran MA, Khan MQ, Salam A, and Ahmad A. Cotton in nonwoven products. Cotton Science and Processing Technology: Gene, Ginning, Garment and Green Recycling. 2020: 305-332. DOI: 10.1007/978-981-15-9169-3_12

Esteve-Turrillas FA, de La Guardia M. Environmental impact of Recover cotton in textile industry. Resour Conserv Recycl. 2017;116:107-115. DOI: 10.1016/j.resconrec.2016.09.034

Doyle JJ,Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem bull; 1987.

Liu K, Muse SV. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics. 2005. 21(9):2128-2129. DOI: 10.1093/bioinformatics/bti282

Kilian B, Graner A. NGS technologies for analyzing germplasm diversity in genebanks. Brief Funct Genomics. 2012;11(1): 38-50. DOI: 10.1093/bfgp/elr046

Van Treuren R, van Hintum TJ. Next-generation genebanking: Plant genetic resources management and utilization in the sequencing era. Plant genet resour. 2014;12(3):298-307. DOI: 10.1017/S1479262114000082

Rafalski JA. Association genetics in crop improvement. Curr Opin Plant Biol. 2010;13(2):174-180.

DOI: 10.1016/j.pbi.2009.12.004

Sun F, Ma J, Shi W, and Yang Y. Genome-wide association analysis revealed genetic variation and candidate genes associated with the yield traits of upland cotton under drought conditions. Front in Plant Sci. 2023;14:1135302.

Venkatesulu S, Makula SP, Satihal MB, Puligundla SK, Srikanth K. Identification of SSR Molecular Markers for Jassid Resistance in Cotton. DOI: 10.18805/ag.D-5740

Sagar T, Kapoor N, and Mahajan R. Microsatellites as potential molecular markers for genetic diversity analysis in plants, in molecular marker techniques: A Potential Approach of Crop Improvement. 2023; Springer 81-101

Zhao Y, Wang H, Chen W, Li Y, Gong H, Sang X, et al. Genetic diversity and population structure of elite cotton (Gossypium hirsutum L.) germplasm revealed by SSR markers. Plant Syst Evol. 2015;301:327-336.

Moiana LD, Vidigal Filho PS, Gonçalves-Vidigal MC, Lacanallo GF, Galván MZ, de Carvalho LP, et al. Genetic diversity and population structure of cotton (Gossypium hirsutum L. race latifolium H.) using microsatellite markers. Afr J Biotechnol. 2012;11(54):11640-11647. DOI: 10.5897/AJB11.4084

Fang DD, Hinze LL, Percy RG, Li P, Deng D, Thyssen GA. microsatellite-based genome-wide analysis of genetic diversity and linkage disequilibrium in Upland cotton (Gossypium hirsutum L.) cultivars from major cotton-growing countries. Euphytica. 2013;191: 391-401. DOI: 10.1007/s10681-013-0886-2

Zhu L, Tyagi P, Kaur B, and Kuraparthy V. Genetic diversity and population structure in elite US and race stock accessions of upland cotton (Gossypium hirsutum). J Cotton Sci. 2019;23(1):38-47.