Applications of Protoplast Fusion in Plant Biotechnology

Gali Adamu Ishaku *

Department of Biotechnology, School of Life Sciences, Modibbo Adama University of Technology, Yola, Nigeria.

Ayuba Kalum Abaka

Department of Biotechnology, School of Life Sciences, Modibbo Adama University of Technology, Yola, Nigeria.

Muhammad Akram

Department of Eastern Medicine, Government College University Faisalabad, Pakistan.

Md. Shariful Islam

Department of Pharmacy, Southeast University, Dhaka, Bangladesh.

*Author to whom correspondence should be addressed.


Plant biotechnology is the deliberate application of biotechnology tools such as protoplast fusion, DNA extraction, plant bioinformatics, PCR and cloning in creating plants with new, improved and desired traits for human benefits. Protoplasts are often referred to as plant cells which the cell wall has been removed and it has many applications in plant biotechnology. Protoplast fusion has been used for centuries but plant biotechnology is a new technology that gives more realistic results in any plants research. The application of plant biotechnology in protoplast fusion will produce new product in plants with wider applications and more realistic results. In this article different applications of protoplast fusion in plant biotechnology was reviewed such as production of useful metabolites, target site mutagenesis, introduction and establishment of disease resistance plants, improvement of food nutrition content, nitrogen-fixation symbioses, production of herbicide resistant plants, insect pest Control and plant-parasitic nematode control in plant for the benefit of humans. The knowledge of protoplast fusion can be use by plant biotechnologist to improve plants trait for human benefits. The application of plant biotechnology is important to any nation’s food security and development.

Keywords: Disease resistance, food security, gene editing, herbicide resistant, nitrogen-fixing symbioses and nutrition content

How to Cite

Ishaku , Gali Adamu, Ayuba Kalum Abaka, Muhammad Akram, and Md. Shariful Islam. 2023. “Applications of Protoplast Fusion in Plant Biotechnology”. Asian Journal of Biotechnology and Genetic Engineering 6 (2):143-54.


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