Asian Journal of Biotechnology and Genetic Engineering 2020-01-14T08:34:52+00:00 Asian Journal of Biotechnology and Genetic Engineering Open Journal Systems <p style="text-align: justify;"><strong>Asian Journal of Biotechnology and Genetic Engineering</strong>&nbsp;aims to publish high-quality papers (<a href="/index.php/AJBGE/general-guideline-for-authors">Click here for Types of paper</a>). The area of interest of AJBGE includes but not restricted to all aspects of Biotechnology, Genetics, Biophysics, Biochemistry, Bioinformatics, Bioenergy, Biosafety, Biosecurity, Bioethics, etc. This journal facilitates the research and wishes to publish papers as long as they are technically correct, scientifically motivated. The journal also encourages the submission of useful reports of negative results. This is a quality controlled,&nbsp;OPEN&nbsp;peer-reviewed, open access INTERNATIONAL journal.</p> Refined Factors in Multi-needle-assisted Transformation of Soybean 2019-12-31T09:34:24+00:00 Biao Zhang Ren-Gao Xue <p>Previous studies have improved<em> Agrobacterium</em>-mediated soybean [<em>Glycine max</em> (L.) Merrill] cotyledonary node method by the development of a simple multi-needle-assisted wounding method using cotyledonary node cells of 1-day-old half seeds as target tissue. The goal of this study was to investigate the factors affecting the efficiency of the multi-needle-assisted transformation of soybean cotyledonary node cells (MNAT). The factors were studied by the GUS activity using a binary vector pCAMBIA1301 containing both a <em>gus</em>-intron gene and a <em>hpt</em> (hygromycin phosphotransferase) selectable marker. All of the factors affecting the transformation efficiency were determined after the 1-day-old half seeds punctured 2 times with the multi-needle. The transformation efficiency based on transient expression of the <em>gus</em> gene was significantly affected by the concentration of antioxidants, density of <em>Agrobacterium</em> suspension, infection time and the concentration of acetosyringone (AS). The frequency of the transformed cotyledonary node cells was also affected by soybean genotypes.</p> 2019-12-31T00:00:00+00:00 ##submission.copyrightStatement## Performance of Tomato (Solanum lycopersicum L.) Genotypes Based on Agro-morphogenic Traits under Drought Condition 2020-01-14T08:34:52+00:00 Rozina Akter Md. Ehsanul Haq Bilkish Begum Naheed Zeba <p>A pot experiment was conducted in the net house of the Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, during November 2013 to March 2014 to observe the performances of fifteen tomato genotypes under three different drought treatments. Two factorial experiments included fifteen tomato genotypes viz. G1 (BD-7759), G2 (BD-7292), G3 (BD-7760), G4 (BD-7258), G5 (BD-7762), G6 (BD-7761), G7 (BD-7289), G8 (BD-7291), G9 (BD-7301), G10 (BARI Tomato-11), G11 (BARI Tomato-9), G12 (BARI Tomato-8), G13 (BARI Tomato-7), G14 (BARI Tomato-3) and G15 (BARI Tomato-2) and three drought treatments, T1 (Control), T2 (30 days withholding of water) and T3 (45 days withholding of water) were outlined in completely randomized design (CRD) with three replications. The results showed that both, the different tomato genotypes and drought treatments had significant influence independently and also in interaction on agro-morphogenic traits of the tomato plant. Almost all traits responded negatively as the drought level increased except days to first flowering, maturity. Considering the yield and yield contributing characters, genotype G4, G5 and G6 showed tolerance at moderate drought stress and G6, G7 and G13 showed tolerance at prolonged and severe drought stress. These genotypes could be recommended to the farmers for cultivation in the drought-prone areas of Bangladesh and also could be used in future hybridization or other gene transfer programs.</p> 2020-12-14T00:00:00+00:00 ##submission.copyrightStatement##