Study of ZNF804A and DISC1 Genes in Iranian Patients with Schizophrenia

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Azizi Faryal
Azizi Parisa
Nemati Fahimeh


Background: Schizophrenia is a complex and chronic psychological disorder characterized by a set of symptoms including illusions, speech and behavioral disorders, and cognitive impairment. Schizophrenia is a complex multiple disorder, and the biggest risk factor for it is a positive family history. The aim of this study was to investigate the relationship between polymorphism of ZNF804A and DISC1 genes in patients with schizophrenia in Iran.

Materials and Methods: In this case-control study, 50 patients with schizophrenia and 50 healthy controls were evaluated. The PCR-RFLP method was used to evaluate single nucleotide polymorphism in both groups of patients and control. For enzymatic digestion of PCR products, rs1344706 and rs6675281 were amplified and digested using MboI and BseLI enzymes at 37 ° C for 16 hours, respectively.

Results: The frequency of TT, GT and GG genotypes for ZNF804A gene in rs1344706 was 26%, 52%, and 22%, respectively, and in healthy subjects 46%, 42%, and 8%, respectively. In the DISC1 gene, the frequency of TT, CT and CC genotypes in the rs6675281 region was 2%, 14%, and 84%, respectively, and 2%, 14%, and 80%, respectively, in healthy subjects or controls, respectively.

Conclusion: Frequency of homozygous GG and heterozygote GT genotypes was 8% and 14% higher than healthy subjects, but the frequency of homozygous TT in healthy subjects was 22% higher than those with schizophrenia for ZNF804A gene in rs1344706 region. However, in case of DISC1 gene, the frequency of TT, CT and CC genotypes in the rs6675281 region was very similar in healthy and healthy subjects, and there was no significant difference between homozygous and heterozygous genotypes. Therefore, the result of our study can be a way to providing suitable information about the disease in order to prepare patients and family and to program adjusted treatment to prevent major injuries.

Heterozygote, genotype, PCR-RFLP, schizophrenia

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Faryal, A., Parisa, A., & Fahimeh, N. (2019). Study of ZNF804A and DISC1 Genes in Iranian Patients with Schizophrenia. Asian Journal of Biotechnology and Genetic Engineering, 2(2), 1-6. Retrieved from
Original Research Article


Patel KR, et al. Schizophrenia: Overview and treatment options. Pharmacy and Therapeutics. 2014;39(9):638-645.

Jablensky A. Epidemiology of schizo-phrenia: The global burden of disease and disability. Eur Arch Psychiatry Clin Neurosci. 2000;250(6):274-85.

Kendler KS, et al. The roscommon family study. I. Methods, diagnosis of probands, and risk of schizophrenia in relatives. Arch Gen Psychiatry. 1993;50(7):527-40.

Cardno AG, et al. Heritability estimates for psychotic disorders: The Maudsley twin psychosis series. Arch Gen Psychiatry. 1999;56(2):162-8.

Trivedi JK. Cognitive deficits in psychiatric disorders: Current status. Indian Journal of Psychiatry. 2006;48(1):10-20.

Barbato A. Psychiatry in transition: Out-comes of mental health policy shift in Italy. Aust N Z J Psychiatry. 1998;32(5):673-9.

Vahia VN. Diagnostic and statistical manual of mental disorders 5: A quick glance. Indian Journal of Psychiatry. 2013; 55(3):220-223.

Gupta S, Kulhara P. What is schizo-phrenia: A neuro developmental or neurodegenerative disorder or a combination of both? A critical analysis. Indian Journal of Psychiatry. 2010;52(1): 21-27.

Lewis DA, Lieberman JA. Catching up on schizophrenia: Natural history and neurobiology. Neuron. 2000;28(2):325- 34.

Gejman PV, Sanders AR, Duan J. The role of genetics in the etiology of schizophrenia. The Psychiatric clinics of North America. 2010;33(1):35-66.

Escudero I, Johnstone M. Genetics of schizophrenia. Curr Psychiatry Rep. 2014; 16(11):502.

del Re EC, et al. Analysis of schizophrenia- related genes and electrophysiological measures reveals ZNF804A association with amplitude of P300b elicited by novel sounds. Translational Psychiatry. 2014; 4(1):e346.

Li M, et al. Allelic differences between Han Chinese and Europeans for functional variants in znf804a and their association with schizophrenia. Am J Psychiatry. 2011; 168(12):1318-25.

Lencz T, et al. A schizophrenia risk gene, ZNF804A, influences neuroanatomical and Neurocognitive Phenotypes. Neuropsycho-pharmacology. 2010;35(11):2284-2291.

Millar JK, et al. Disruption of two novel genes by a translocation co-segregating with schizophrenia. Hum Mol Genet. 2000; 9(9):1415-23.

Leliveld SR, et al. Insolubility of disrupted-in-schizophrenia 1 disrupts oligomer-dependent interactions with nuclear distribution element 1 and is associated with sporadic mental disease. The Journal of Neuroscience. 2008;28(15):3839.

Badner JA, Gershon ES. Meta-analysis of whole-genome linkage scans of bipolar disorder and schizophrenia. Mol Psychiatry. 2002;7(4):405-11.

Messias E, Chen CY, Eaton WW. Epidemiology of schizophrenia: Review of findings and myths. The Psychiatric Clinics of North America. 2007;30(3):323-338.

Tao R, et al. Expression of ZNF804A in human brain and alterations in schizo-phrenia, bipolar disorder, and major depressive disorder: A novel transcript fetally regulated by the psychosis risk variant rs1344706. JAMA Psychiatry. 2014;71(10):1112-20.

Chang H, Xiao X, Li M. The schizophrenia risk gene ZNF804A: Clinical associations, biological mechanisms and neuronal functions. Mol Psychiatry. 2017;22(7):944-953.

Pourtalebi AHA, Fallah J, Bidkhori GH, Investigation of the DISC gene 1 poly-morphism involved in the development of the cortex and the development of the neural network in schizophrenic patients: the first study on Iranian population. The Quarterly Journal of Animal Physiology and Development. 2015;8(3):45-52.

Morris SE, et al. Dissociation of response and feedback negativity in schizophrenia: Electrophysiological and computational evidence for a deficit in the representation of value. Frontiers in Human Neuroscience. 2011;5:123.